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KMID : 0379519990150030297
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한국독성학회지
1999 Volume.15 No. 3 p.297 ~ p.306
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A Comparison for Antioxidant Activity of Carnosine and Related Compounds in Several Model Systems
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Lee Beom-Jun
Kang Kyung-Sun
Lee Yong-Soon
Nam Sang-Yoon
Kim Young-Chul
Cho Myung-Haing
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Abstract
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The antioxidant activities of carnosine and related compounds (CRCs) including anserine and homocarnosine have been reported mainly using phosphatidylcholine (PC) liposomes. In this study, the antioxidant activities of these compounds and their components, L-histidine and β-alanine were compared using several different in vitro model systems. In either Cu (Ⅱ) or Fe (Ⅲ) + ascorbic acid (AA)-catalyzed deoxyribose system, carnosine, homocarnosine, anserine, and histidine showed a strong inhibitory effect on deoxyribose degradation, as measured by thiobarbituric acid-reactive substances for OH scavenging activity. The inhibitory effect of carnosine, homocarnosine, and anserine was greater in the Cu (Ⅱ) system than the Fe (Ⅲ) system. Although carnosine and anserine stimulated H202 formation in the presence of 10 mM glucose and 10 m M Cu (Ⅱ), they simultaneously inhibited deoxyribose degradation. Histidine-containing dipeptides were very effective for inhibition of lipid peroxidation in PC liposomes, as measured by malondialdehyde, lipid peroxide, and conjugated diene. Among the measurements, there was a correlationship as expected. Carnosine reduced Cu (Ⅱ) to Cu (Ⅰ) and the reducing potential of carnosine was 1/10 of that of AA. CRCs strongly inhibited HOCl-induced AA oxidation but only carnosine and anserine inhibited HOCl-induced inactivation of a-antitrypsin activity in a preincubation time-dependent manner. Carnosine, homocarnosine, anserine, and histidine inhibited Fe (Ⅲ) + AA-induced bovine erythrocyte hemolysis and methemoglobin formation. These results indicate that carnosine, homocarnosine, and anserine have a comparable antioxidant activity, probably due to the histidine moiety of these compounds.
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KEYWORD
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antioxidant, ascorbic acid, carnosine and related compounds, hemolysis, hypochlorite, lipid peroxidation
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